Utility of Alternative Promoters for Foreign Gene Expression Using the Baculovirus Expression Vector System.

ABSTRACT: The baculovirus expression vector system (BEVS) is a widely used platform for recombinant protein production for use in a wide variety of applications. Of particular interest is production of virus-like particles (VLPs), which consist of multiple viral proteins that self-assemble in strict stoichiometric ratios to mimic the structure of a virus but lacks its genetic material, while a significant amount of effort has been spent on optimizing expression ratios by co-infecting cells with multiple recombinant BEVs and modulating different process parameters, co-expressing multiple foreign genes from a single rBEV may offer more promise. However, there is currently a lack of promoters available with which to optimize co-expression of each foreign gene. To address this, previously published tran

SUBMITTER: Bruder MR 

PROVIDER: S-EPMC9786725 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature