Proteomic profiling of idiopathic Parkinson's disease primary patient cells by SWATH-MS.

ABSTRACT:

Purpose

Parkinson's disease (PD) is the second most prevalent neurodegenerative disease. It is generally diagnosed clinically after the irreversible loss of dopaminergic neurons and no general biomarkers currently exist. To gain insight into the underlying cellular causes of PD we aimed to quantify the proteomic differences between healthy control and PD patient cells.

Experimental design

Sequential Window Acquisition of all THeoretical Mass Spectra was performed on primary cells from healthy controls and PD patients.

Results

In total, 1948 proteins were quantified and 228 proteins were significantly differentially expressed in PD patient cells. In PD patient cells, we identified seven significantly increased proteins involved in the unfolded protein response (UPR) an

SUBMITTER: Wood SA 

PROVIDER: S-EPMC9787017 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature