Peripheral blood mononuclear cells-expressed miRNA profiles derived from children with metabolic-associated fatty liver disease and insulin resistance.

ABSTRACT:

Background

miRNA have been proposed as potential biomarkers of metabolic diseases.

Objectives

To identify potential miRNA biomarkers of early metabolic-associated fatty liver disease (MAFLD) and/or insulin resistance (IR) in preadolescent children.

Methods

A total of 70 preadolescents, aged 8.5-12 years old participated in the study. Hepatic fat was assessed by magnetic resonance imaging. Fasting blood biochemical parameters were measured and HOMA-IR calculated. Peripheral blood mononuclear cells (PBMC)-derived miRNA profiles associated with MAFLD (≥5.5% hepatic fat) and IR (HOMA-IR ≥2.5) were identified using untargeted high-throughput miRNAs sequencing (RNA-seq).

Results

A total of 2123 PBMC-derived miRNAs were identified in children with (21.4%) or without M

SUBMITTER: Oses M 

PROVIDER: S-EPMC9787576 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature