Extracellular vesicles from equine mesenchymal stem cells decrease inflammation markers in chondrocytes in vitro.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) have been used therapeutically in equine medicine. MSCs release extracellular vesicles (EVs), which affect cell processes by inhibiting cell apoptosis and regulating inflammation. To date, little is known about equine EVs and their regenerative properties.

Objectives

To characterise equine MSC-derived extracellular vesicles (EVs) and evaluate their effect on equine chondrocytes treated with pro-inflammatory cytokines in vitro.

Study design

In vitro experiments with randomised complete block design.

Methods

Mesenchymal stem cells from bone marrow, adipose tissue, and synovial fluid were cultured in vitro. The MSC culture medium was centrifuged and filtered. Isolated particles were analysed for size and concentration (tota

SUBMITTER: Arevalo-Turrubiarte M 

PROVIDER: S-EPMC9787580 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature