Molecular Characterization and Genetic Variation in Ceratocystis fimbriata Ell. and Halst. on Pomegranate

ABSTRACT: Fifteen isolates of Ceratocystis fimbriata collected from different locations in Karnataka were characterized using ITS gene technology. It produced an amplification size of 600−650 bp, which indicated that all the isolates belong to the genus Ceratocystis, thus confirming the identity of the pathogenic isolates. To test genetic variability, isolates were analyzed using microsatellite markers. An UPGMA dendrogram for genetic variation among the isolates showed that all the isolates fell into two major clusters. The first cluster consisted of isolate Cf-10 and the second cluster was further divided into two sub-clusters. Sub-cluster one consisted of isolate Cf-2. Sub-cluster two was again divided into five groups. The first group included isolate Cf-13, the second group consisted of isolate

SUBMITTER: Erakyathappa NS 

PROVIDER: S-EPMC9787934 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature