Heterologous Expression and Immunogenic Potential of the Most Abundant Phospholipase A₂ from Coral Snake Micrurus dumerilii to Develop Antivenoms.

ABSTRACT: Micrurus dumerilii is a coral snake of clinic interest in Colombia. Its venom is mainly composed of phospholipases A₂ being MdumPLA₂ the most abundant protein. Nevertheless, Micrurus species produce a low quantity of venom, which makes it difficult to produce anticoral antivenoms. Therefore, in this work, we present the recombinant expression of MdumPLA₂ to evaluate its biological activities and its immunogenic potential to produce antivenoms. For this, a genetic construct rMdumPLA₂ was cloned into the pET28a vector and expressed heterologously in bacteria. His-rMdumPLA₂ was extracted from inclusion bodies, refolded in vitro, and isolated using affinity and RP-HPLC chromatography. His-rMdumPLA₂ was shown to hav

SUBMITTER: Romero-Giraldo LE 

PROVIDER: S-EPMC9788014 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature