Multifunctional Protein A Is the Only Viral Protein Required for Nodavirus RNA Replication Crown Formation.

ABSTRACT: Positive-strand RNA virus RNA genome replication occurs in membrane-associated RNA replication complexes (RCs). Nodavirus RCs are outer mitochondrial membrane invaginations whose necked openings to the cytosol are "crowned" by a 12-fold symmetrical proteinaceous ring that functions as the main engine of RNA replication. Similar protein crowns recently visualized at the openings of alphavirus and coronavirus RCs highlight their broad conservation and functional importance. Using cryo-EM tomography, we earlier showed that the major nodavirus crown constituent is viral protein A, whose polymerase, RNA capping, membrane interaction and multimerization domains drive RC formation and function. Other viral proteins are strong candidates for unassigned EM density in the crown. RNA-binding RNAi inh

SUBMITTER: den Boon JA 

PROVIDER: S-EPMC9788154 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature