The role of filamentation in activation and DNA sequence specificity of the sequence-specific endonuclease SgrAI.

ABSTRACT: Filament formation by metabolic, biosynthetic, and other enzymes has recently come into focus as a mechanism to fine-tune enzyme activity in the cell. Filamentation is key to the function of SgrAI, a sequence-specific DNA endonuclease that has served as a model system to provide some of the deepest insights into the biophysical characteristics of filamentation and its functional consequences. Structure-function analyses reveal that, in the filamentous state, SgrAI stabilizes an activated enzyme conformation that leads to accelerated DNA cleavage activity and expanded DNA sequence specificity. The latter is thought to be mediated by sequence-specific DNA structure, protein-DNA interactions, and a disorder-to-order transition in the protein, which collectively affect the relative stabilities

SUBMITTER: Lyumkis D 

PROVIDER: S-EPMC9788392 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature