Analysis of Proteins and Peptides of Highly Purified CD9⁺ and CD63⁺ Horse Milk Exosomes Isolated by Affinity Chromatography.

ABSTRACT: Exosomes are nanovesicles with a 40-150 nm diameter and are essential for communication between cells. Literature data suggest that exosomes obtained from different sources (cell cultures, blood plasma, urea, saliva, tears, spinal fluid, milk) using a series of centrifugations and ultracentrifugations contain hundreds and thousands of different protein and nucleic acid molecules. However, most of these proteins are not an intrinsic part of exosomes; instead, they co-isolate with exosomes. Using consecutive ultracentrifugation, gel filtration, and affinity chromatography on anti-CD9- and anti-CD63-Sepharoses, we isolated highly purified vesicle preparations from 18 horse milk samples. Gel filtration of the initial preparations allowed us to remove co-isolating proteins and their complexes a

SUBMITTER: Sedykh SE 

PROVIDER: S-EPMC9788572 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature