DNA barcoding and gene expression recording reveal the presence of cancer cells with unique properties during tumor progression.

ABSTRACT: Tumors comprise diverse cancer cell populations with specific capabilities for adaptation to the tumor microenvironment, resistance to anticancer treatments, and metastatic dissemination. However, whether these populations are pre-existing in cancer cells or stochastically appear during tumor growth remains unclear. Here, we show the heterogeneous behaviors of cancer cells regarding response to anticancer drug treatments, formation of lung metastases, and expression of transcription factors related to cancer stem-like cells using a DNA barcoding and gene expression recording system. B16F10 cells maintained clonal diversity after treatment with HVJ-E, a UV-irradiated Sendai virus, and the anticancer drug dacarbazine. PBS treatment of the primary tumor and intravenous injection of B16F10 cel

SUBMITTER: Umeki Y 

PROVIDER: S-EPMC9789022 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature