Biodegradation of bovine spongiform encephalopathy prions in compost.

ABSTRACT: To reduce the transmission risk of bovine spongiform encephalopathy prions (PrP^BSE), specified risk materials (SRM) that can harbour PrP^BSE are prevented from entering the feed and food chains. As composting is one approach to disposing of SRM, we investigated the inactivation of PrP^BSE in lab-scale composters over 28 days and in bin composters over 106-120 days. Lab-scale composting was conducted using 45 kg of feedlot manure with and without chicken feathers. Based on protein misfolding cyclic amplification (PMCA), after 28 days of composting, PrP^BSE seeding activity was reduced by 3-4 log₁₀ with feathers and 3 log₁₀ without. Bin composters were constructed using ~ 2200 kg feedlot manure and repeated in 2017 and 2018. PMCA

SUBMITTER: Xu S 

PROVIDER: S-EPMC9789035 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature