A novel feature for monitoring the enzymatic harvesting process of adherent cell cultures based on lens-free imaging.

ABSTRACT: Adherent cell cultures are often dissociated from their culture vessel (and each other) through enzymatic harvesting, where the detachment response is monitored by an operator. However, this approach is lacking standardisation and reproducibility, and prolonged exposure or too high concentrations can affect the cell's viability and differentiation potential. Quantitative monitoring systems are required to characterise the cell detachment response and objectively determine the optimal time-point to inhibit the enzymatic reaction. State-of-the-art methodologies rely on bulky imaging systems and/or features (e.g. circularity) that lack robustness. In this study, lens-free imaging (LFI) technology was used to develop a novel cell detachment feature. Seven different donors were cultured and sub

SUBMITTER: Deckers T 

PROVIDER: S-EPMC9789138 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature