Adsorption of a water-soluble molecular rotor fluorescent probe on hydrophobic surfaces.

ABSTRACT: Environmentally sensitive molecular rotors are widely used to probe the local molecular environment in e.g. polymer solutions, polymer glasses, and biological systems. These applications make it important to understand its fluorescence properties in the vicinity of a solid surface, since fluorescence microscopy generically employs cover slides, and measurements are often done in its immediate vicinity. Here, we use a confocal microscope to investigate the fluorescence of (4-DASPI) in glycerol/water solutions close to the interface using hydrophilic or hydrophobic cover slips. Despite the dye's high solubility in water, the observed lengthening of the fluorescence lifetime close to the hydrophobic surface, implies a surprising affinity of the dye with the surface. Because the homogeneous so

SUBMITTER: Mirzahossein E 

PROVIDER: S-EPMC9789158 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature