Gene expression profiles of ischemic stroke clots retrieved by mechanical thrombectomy are associated with disease etiology.

ABSTRACT:

Background

Determining stroke etiology is crucial for secondary prevention, but intensive workups fail to classify ~30% of strokes that are cryptogenic.

Objective

To examine the hypothesis that the transcriptomic profiles of clots retrieved during mechanical thrombectomy are unique to strokes of different subtypes.

Methods

We isolated RNA from the clots of 73 patients undergoing mechanical thrombectomy. Samples of sufficient quality were subjected to 100-cycle, paired-end RNAseq, and transcriptomes with less than 10 million unique reads were excluded from analysis. Significant differentially expressed genes (DEGs) between subtypes (defined by the Trial of Org 10 172 in Acute Stroke Treatment) were identified by expression analysis in edgeR. Gene ontology enrichment an

SUBMITTER: Tutino VM 

PROVIDER: S-EPMC9789205 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature