Methionine regulates self-renewal, pluripotency, and cell death of GIC through cholesterol-rRNA axis.

ABSTRACT:

Background

Glioma-initiating cells (GICs) are the source of glioma cells that can self-renew, have pluripotency, and are treatment-resistant, so are the starting point for relapse and eventual death despite multimodality therapy. L-[methyl-¹¹C] methionine PET has observed high accumulation at the time of recurrence, it is important to understand the mechanism of tumor cell activation caused by the reorganization of methionine metabolism.  METHODS: We cultured cells in methionine-deprived culture medium for comprehensive analysis. Based on the obtained results, the possible target molecules were chemically inhibited and the respective markers were analyzed.

Results

Methionine depletion markedly decreased proliferation and increased cell death of GICs. Decreased S-a

SUBMITTER: Yokogami K 

PROVIDER: S-EPMC9789638 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature