Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation.

ABSTRACT: Classic microsurgical techniques, such as those used in the early 1900s by Mangold and Spemann, have been instrumental in advancing our understanding of embryonic development. However, these techniques are highly specialized, leading to issues of inter-operator variability. Here we introduce a user-friendly robotic microsurgery platform that allows precise mechanical manipulation of soft tissues in zebrafish embryos. Using our platform, we reproducibly targeted precise regions of tail explants, and quantified the response in real-time by following notochord and presomitic mesoderm (PSM) morphogenesis and segmentation clock dynamics during vertebrate anteroposterior axis elongation. We find an extension force generated through the posterior notochord that is strong enough to buckle the stru

SUBMITTER: Ozelci E 

PROVIDER: S-EPMC9789989 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature