Combination of genetic engineering and random mutagenesis for improving production of raw-starch-degrading enzymes in Penicillium oxalicum.

ABSTRACT:

Background

Raw starch-degrading enzyme (RSDE) is applied in biorefining of starch to produce biofuels efficiently and economically. At present, RSDE is obtained via secretion by filamentous fungi such as Penicillium oxalicum. However, high production cost is a barrier to large-scale industrial application. Genetic engineering is a potentially efficient approach for improving production of RSDE. In this study, we combined genetic engineering and random mutagenesis of P. oxalicum to enhance RSDE production.

Results

A total of 3619 mutated P. oxalicum colonies were isolated after six rounds of ethyl methanesulfonate and Co⁶⁰-γ-ray mutagenesis with the strain A2-13 as the parent strain. Mutant TE4-10 achieved the highest RSDE production of 218.6 ± 3.8 U/mL with raw ca

SUBMITTER: Zhao S 

PROVIDER: S-EPMC9790131 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature