First isolation and whole genome characterization of porcine deltacoronavirus from pigs in Peru.

ABSTRACT: Porcine deltacoronavirus is a newly emergent enteric pathogen affecting swine farms worldwide. It has been detected in several countries in Europe, Asia and North America; yet, it has not been reported in South America. In November 2019, an enteric disease outbreak in a pig farm located in San Martin, Peru, was reported along with submission of three intestinal samples from pigs who succumbed to the disease. Samples were processed for molecular detection by qRT-PCR, viral isolation and further sequencing analysis. A taqman-based RT-PCR was performed to differentiate among the most relevant swine enteric coronaviruses described to date. All samples were positive to porcine deltacoronavirus with a cycle threshold (Ct) value between 9 and 14, revealing a high viral load, while testing negativ

SUBMITTER: More-Bayona JA 

PROVIDER: S-EPMC9790302 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature