Oxidized LDL is stable in human serum under extended thawed-state conditions ranging from -20 °C to room temperature.

ABSTRACT:

Introduction

Oxidized LDL (oxLDL) is formed by the spontaneous reaction between aldehyde byproducts of lipid peroxidation and lysine residues of apolipoprotein B within LDL. Clinically, oxLDL is used as a marker of coronary artery disease and predictor of metabolic syndrome risk. Despite its popularity as a clinical marker, no systematic studies of oxLDL stability, in which serum or plasma has been pre-analytically exposed to an array of different time and temperature conditions, have been carried out.

Objective

To systematically evaluate the stability of oxLDL in human serum samples exposed to thawed conditions (> -30 °C) for varying periods of time while monitoring a second protein/small molecule redox system as a positive control for non-enzymatic biomolecular activity.

SUBMITTER: Jehanathan N 

PROVIDER: S-EPMC9791165 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature