Network Topology Can Explain Differences in Pleiotropy Between Cis- and Trans-regulatory Mutations.

ABSTRACT: A mutation's degree of pleiotropy (i.e., the number of traits it alters) is predicted to impact the probability of the mutation being detrimental to fitness. For mutations that impact gene expression, mutations acting in cis have been hypothesized to generally be less pleiotropic than mutations affecting the same gene's expression in trans, suggesting that cis-regulatory mutations should be less deleterious and more likely to fix over evolutionary time. Here, we use expression and fitness data from Saccharomyces cerevisiae gene deletion strains to test these hypotheses. By treating deletion of each gene as a cis-regulatory mutation affecting its own expression and deletions of other genes affecting expression of this focal gene as trans-regulatory mutations, we find that cis-acting mutatio

SUBMITTER: Vande Zande P 

PROVIDER: S-EPMC9791367 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature