TIGIT blockade repolarizes AML-associated TIGIT⁺ M2 macrophages to an M1 phenotype and increases CD47-mediated phagocytosis.

ABSTRACT:

Background

Leukemia-associated macrophages (LAMs) represent an important cell population within the tumor microenvironment, but little is known about the phenotype, function, and plasticity of these cells. The present study provides an extensive characterization of macrophages in patients with acute myeloid leukemia (AML).

Methods

The phenotype and expression of coregulatory markers were assessed on bone marrow (BM)-derived LAM populations, using multiparametric flow cytometry. BM and blood aspirates were obtained from patients with newly diagnosed acute myeloid leukemia (pAML, n=59), patients in long-term remission (lrAML, n=8), patients with relapsed acute myeloid leukemia (rAML, n=7) and monocyte-derived macrophages of the blood from healthy donors (HD, n=17). LAM subpopu

SUBMITTER: Brauneck F 

PROVIDER: S-EPMC9791419 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature