Cell-free metabolic engineering enables selective biotransformation of fatty acids to value-added chemicals.

ABSTRACT: Fatty acid-derived products such as alkanes, fatty aldehydes, and fatty alcohols have many applications in the chemical industry. These products are predominately produced from fossil resources, but their production processes are often not environmentally friendly. While microbes like Escherichia coli have been engineered to convert fatty acids to corresponding products, the design and optimization of metabolic pathways in cells for high productivity is challenging due to low mass transfer, heavy metabolic burden, and intermediate/product toxicity. Here, we describe an E. coli-based cell-free protein synthesis (CFPS) platform for in vitro conversion of long-chain fatty acids to value-added chemicals with product selectivity, which can also avoid the above issues when u

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9791597 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature