Development of an expression-tunable multiple protein synthesis system in cell-free reactions using T7-promoter-variant series

ABSTRACT: Abstract New materials with a low environmental load are expected to be generated through synthetic biology. To widely utilize this technology, it is important to create cells with designed biological functions and to control the expression of multiple enzymes. In this study, we constructed a cell-free evaluation system for multiple protein expression, in which synthesis is controlled by T7 promoter variants. The expression of a single protein using the T7 promoter variants showed the expected variety in expression levels, as previously reported. We then examined the expression levels of multiple proteins that are simultaneously produced in a single well to determine whether they can be predicted from the promoter activity values, which were defined from the isolated protein expression lev

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PROVIDER: S-EPMC9791696 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature