Neutrophil extracellular traps induce the bone erosion of gout.

ABSTRACT:

Objective

To investigate the relationships between monosodium urate (MSU) crystals -induced neutrophil extracellular traps (NETs) and bone erosion in gout.

Methods

Animal models were used to study the relationship between NETs induced by MSU crystals and bone erosion. Neutrophils were treated with MSU crystals to induce NETs. The osteoblasts-like cells (OB) were then treated with NETs, and the supernatant was co-incubated with osteoclasts-like cells (OC). The NETs were digested with DNase, and the neutrophil elastase (NE) was inhibited with sivelestat sodium. Cell viability, mRNA, and protein expression were also assessed.

Results

After treating OB with NETs, the cell viability decreased. Yet, after digesting the DNA and inhibiting NE, the viability was moderately imp

SUBMITTER: Jia E 

PROVIDER: S-EPMC9791768 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature