Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335.

ABSTRACT: Pseudomonas syringae pv. savastanoi NCPPB 3335 is the causal agent of olive knot disease and contains three virulence plasmids: pPsv48A (pA), 80 kb; pPsv48B (pB), 45 kb, and pPsv48C (pC), 42 kb. Here we show that pB contains a complete MPF_T (previously type IVA secretion system) and a functional origin of conjugational transfer adjacent to a relaxase of the MOB_P family; pC also contains a functional oriT-MOB_P array, whereas pA contains an incomplete MPF_I (previously type IVB secretion system), but not a recognizable oriT. Plasmid transfer occurred on solid and in liquid media, and on leaf surfaces of a non-host plant (Phaseolus vulgaris) with high (pB) or moderate frequency (pC); pA was transferred only occasionally af

SUBMITTER: Anorga M 

PROVIDER: S-EPMC9791958 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature