Parallel deployment of passive and composite samplers for surveillance and variant profiling of SARS-CoV-2 in sewage.

ABSTRACT: Wastewater-based epidemiology during the COVID-19 pandemic has proven useful for public health decision-making but is often hampered by sampling methodology constraints, particularly at the building- or neighborhood-level. Time-weighted composite samples are commonly used; however, autosamplers are expensive and can be affected by intermittent flows in sub-sewershed contexts. In this study, we compared time-weighted composite, grab, and passive sampling via Moore swabs, at four locations across a college campus to understand the utility of passive sampling. After optimizing the methods for sample handling and processing for viral RNA extraction, we quantified SARS-CoV-2 N1 and N2, as well as a fecal strength indicator, PMMoV, by ddRT-PCR and applied tiled amplicon sequencing of the SARS-Co

SUBMITTER: Cha G 

PROVIDER: S-EPMC9792180 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature