The BBSome regulates mitochondria dynamics and function.

ABSTRACT:

Objective

The essential role of mitochondria in regulation of metabolic function and other physiological processes has garnered enormous interest in understanding the mechanisms controlling the function of this organelle. We assessed the role of the BBSome, a protein complex composed of eight Bardet-Biedl syndrome (BBS) proteins, in the control of mitochondria dynamic and function.

Methods

We used a multidisciplinary approach that include CRISPR/Cas9 technology-mediated generation of a stable Bbs1 gene knockout hypothalamic N39 neuronal cell line. We also analyzed the phenotype of BBSome deficient mice in presence or absence of the gene encoding A-kinase anchoring protein 1 (AKAP1).

Results

Our data show that the BBSome play an important role in the regulation of mito

SUBMITTER: Guo DF 

PROVIDER: S-EPMC9792363 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature