Synapse integrity and function: Dependence on protein synthesis and identification of potential failure points.

ABSTRACT: Synaptic integrity and function depend on myriad proteins - labile molecules with finite lifetimes that need to be continually replaced with freshly synthesized copies. Here we describe experiments designed to expose synaptic (and neuronal) properties and functions that are particularly sensitive to disruptions in protein supply, identify proteins lost early upon such disruptions, and uncover potential, yet currently underappreciated failure points. We report here that acute suppressions of protein synthesis are followed within hours by reductions in spontaneous network activity levels, impaired oxidative phosphorylation and mitochondrial function, and, importantly, destabilization and loss of both excitatory and inhibitory postsynaptic specializations. Conversely, gross impairments in pre

SUBMITTER: Cohen LD 

PROVIDER: S-EPMC9792512 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature