Intact provirus and integration sites analysis in acute HIV-1 infection and changes after one year of early antiviral therapy.

ABSTRACT:

Background and objectives

HIV-1 provirus integration in host genomes provides a lifelong reservoir of virally infected cells. Although not able to generate viral progeny, the expression of defective proviruses has been associated with activation. Provirus integration may influence host gene transcription and shifts may occur during disease progression or antiretroviral therapy (ART). The study aimed to analyze intact/defective provirus and sites of provirus integration in acute infections: changes after 48 weeks of early therapy were also evaluated.

Methods

DNA from peripheral blood lymphomonocytes of 8 acute HIV-1 infections at serodiagnosis (T0) and after 48 weeks of therapy (T1) was used to quantify intact and defective provirus by digital-droplet PCR and to analyze provi

SUBMITTER: Rozera G 

PROVIDER: S-EPMC9792883 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature