Extraction and Oxford Nanopore sequencing of genomic DNA from filamentous Actinobacteria.
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ABSTRACT:
SUBMITTER: Alvarez-Arevalo M
PROVIDER: S-EPMC9792953 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Publications
Extraction and Oxford Nanopore sequencing of genomic DNA from filamentous Actinobacteria.
Alvarez-Arevalo Maria M Sterndorff Eva Baggesgaard EB Faurdal David D Jørgensen Tue Sparholt TS Mourched Anna-Sophie AS Vuksanovic Oliwia O Saha Subhasish S Weber Tilmann T
STAR protocols 20221216 1
Actinomycetota (Actinobacteria) is an ecologically and industrially important phylum which is challenging to extract pure high-molecular-weight (HMW) DNA from. This protocol provides a parallelized, cost-effective, and straightforward approach for consistently extracting pure HMW DNA using modified non-toxic commercial kits suitable for higher throughput applications. We further provide a workflow for sequencing and assembly of complete genomes using an optimized Oxford Nanopore rapid barcoding ...[more]
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