Detection of AmpC β-lactamases in gram-negative bacteria.

ABSTRACT: AmpC β-lactamase genes are clinically important because they often confer resistance to most β-lactams other than 4th-generation cephalosporins and carbapenems. However, traditional and existing detection methods are expensive, labor-intensive and range-limited. We established an efficient multiplex PCR method to simultaneously identify six families of ampC β-lactamase genes, ACC, EBC, CIT, DHA, MOX and FOX, and evaluated the sensitivity and specificity of this assay. The multiplex method could accurately identify ACC, EBC, CIT, DHA, MOX and FOX variants among a total of 175 ampC β-lactamase genes. The minimum concentration of genomic DNA that could be detected was 1.0×10³ copies/μL. We subsequently used this method to analyze 2 Salmonella spp. with carrying

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC9793173 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature