Large-conductance calcium-activated K⁺ channels, rather than K_ATP channels, mediate the inhibitory effects of nitric oxide on mouse lymphatic pumping.

ABSTRACT:

Background and purpose

K_ATP channels are negative regulators of lymphatic vessel excitability and contractility and are proposed to be targets for immune cell products that inhibit lymph transport. Previous studies in rat and guinea pig mesenteric lymphatics found that NO-mediated inhibition of lymphatic contraction was prevented or reversed by the K_ATP channel inhibitor, glibenclamide. We revisited this hypothesis using mouse lymphatic vessels and K_ATP channel knockout mice.

Experimental approach

Mouse popliteal lymphatics were isolated, and contractility was assessed using pressure myography. K⁺ channel expression was determined by PCR analysis of FACS-purified lymphatic smooth muscle cells.

Key results

The NO-producing ag

SUBMITTER: Kim HJ 

PROVIDER: S-EPMC9793343 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature