Ontology highlight
ABSTRACT:
SUBMITTER: Maus H
PROVIDER: S-EPMC9793963 | biostudies-literature | 2023 Jan
REPOSITORIES: biostudies-literature
Maus Hannah H Hinze Gerald G Hammerschmidt Stefan Josef SJ Basché Thomas T Schirmeister Tanja T
Protein science : a publication of the Protein Society 20230101 1
Ligand binding to proteins often is accompanied by conformational transitions. Here, we describe a competition assay based on single molecule Förster resonance energy transfer (smFRET) to investigate the ligand-induced conformational changes of the dengue virus (DENV) NS2B-NS3 protease, which can adopt at least two different conformations. First, a competitive ligand was used to stabilize the closed conformation of the protease. Subsequent addition of the allosteric inhibitor reduced the fractio ...[more]