Fusobacterium nucleatum induces colon anastomosis leak by activating epithelial cells to express MMP9.

ABSTRACT:

Background

Despite advances in anastomotic techniques and perioperative care, the incidence of anastomotic leak (AL) has not substantially decreased over time. Although it is known that AL etiology is multifactorial and the mechanisms involved remain unclear, there is accumulating evidence pointing at AL related to gut microbiota.

Method

We firstly performed a clinical study to analyze the gut microbiota between colorectal cancer patients who developed AL and those who did not (nAL) using 16S-rRNA sequencing and quantitative real-time PCR to identify AL risk bacterial taxa. Then we built a rat anastomosis model and performed a bacteria transplantation to ensure the cause-effect relationship. The anastomotic healing score was used to evaluate the healing of anastomosis. In ad

SUBMITTER: Shi S 

PROVIDER: S-EPMC9794562 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature