SCON-a Short Conditional intrON for conditional knockout with one-step zygote injection.

ABSTRACT: The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.

SUBMITTER: Wu SS 

PROVIDER: S-EPMC9794761 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature