FLIP-based autophagy-detecting technique reveals closed autophagic compartments.

ABSTRACT: Autophagy results in the degradation of cytosolic components via two major membrane deformations. First, the isolation membrane sequesters components from the cytosol and forms autophagosomes, by which open structures become closed compartments. Second, the outer membrane of the autophagosomes fuses with lysosomes to degrade the inner membrane and its contents. The efficiency of the latter degradation process, namely autophagic flux, can be easily evaluated using lysosomal inhibitors, whereas the dynamics of the former process is difficult to analyze because of the challenges in identifying closed compartments of autophagy (autophagosomes and autolysosomes). To resolve this problem, we here developed a method to detect closed autophagic compartments by applying the FLIP technique, and name

SUBMITTER: Sakurai HT 

PROVIDER: S-EPMC9794774 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature