Predicting stress response and improved protein overproduction in Bacillus subtilis.

ABSTRACT: Bacillus subtilis is a well-characterized microorganism and a model for the study of Gram-positive bacteria. The bacterium can produce proteins at high densities and yields, which has made it valuable for industrial bioproduction. Like other cell factories, metabolic modeling of B. subtilis has discovered ways to optimize its metabolism toward various applications. The first genome-scale metabolic model (M-model) of B. subtilis was published more than a decade ago and has been applied extensively to understand metabolism, to predict growth phenotypes, and served as a template to reconstruct models for other Gram-positive bacteria. However, M-models are ill-suited to simulate the production and secretion of proteins as well as their proteomic response to stress. Thus, a new generation of me

SUBMITTER: Tibocha-Bonilla JD 

PROVIDER: S-EPMC9794813 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature