Altered serum human cytomegalovirus microRNA levels are common and closely associated with the inflammatory status in patients with fever.

ABSTRACT:

Background

Fever has a complicated etiology, and diagnosing its causative factor is clinically challenging. Human cytomegalovirus (HCMV) infection causes various diseases. However, the clinical relevance, prevalence, and significance of HCMV microRNAs (miRNA) in association with fever remain unclear. In the present study, we analyzed the HCMV miRNA expression pattern in the serum of patients with fever and evaluate its clinical associations with occult HCMV infection status in immune disorders.

Methods

We included serum samples from 138 patients with fever and 151 age-gender-matched controls in this study. First, the serum levels of 24 HCMV miRNAs were determined using a hydrolysis probe-based stem-loop quantitative reverse transcription polymerase chain reaction (RT-qPCR) a

SUBMITTER: Wang C 

PROVIDER: S-EPMC9795028 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature