In vitro-derived medium spiny neurons recapitulate human striatal development and complexity at single-cell resolution.

ABSTRACT: Stem cell engineering of striatal medium spiny neurons (MSNs) is a promising strategy to understand diseases affecting the striatum and for cell-replacement therapies in different neurological diseases. Protocols to generate cells from human pluripotent stem cells (PSCs) are scarce and how well they recapitulate the endogenous fetal cells remains poorly understood. We have developed a protocol that modulates cell seeding density and exposure to specific morphogens that generates authentic and functional D1- and D2-MSNs with a high degree of reproducibility in 25 days of differentiation. Single-cell RNA sequencing (scRNA-seq) shows that our cells can mimic the cell-fate acquisition steps observed in vivo in terms of cell type composition, gene expression, and signaling pathways. Fina

SUBMITTER: Conforti P 

PROVIDER: S-EPMC9795363 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature