Suppression of porcine hemagglutinating encephalomyelitis virus replication by resveratrol.

ABSTRACT:

Background

Porcine hemagglutinating encephalomyelitis virus (PHEV), a member of the genus Betacoronavirus, is the causative agent of neurological disease in pigs. No effective therapeutics are currently available for PHEV infection. Resveratrol has been shown to exert neuroprotective and antiviral effects. Here resveratrol was investigated for its ability to inhibit PHEV replication in nerve cells and central nervous system tissues.

Methods

Anti-PHEV effect of resveratrol was evaluated using an in vitro cell-based PHEV infection model and employing a mouse PHEV infection model. The collected cells or tissues were used for quantitative PCR analysis, western blot analysis, or indirect immunofluorescence assay. The supernatants were collected to quantify viral loads by TCID

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9795454 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature