Small molecule-mediated rapid maturation of human induced pluripotent stem cell-derived cardiomyocytes.

ABSTRACT:

Background

Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (iPSC-CMs) do not display all hallmarks of mature primary cardiomyocytes, especially the ability to use fatty acids (FA) as an energy source, containing high mitochondrial mass, presenting binucleation and increased DNA content per nuclei (polyploidism), and synchronized electrical conduction. This immaturity represents a bottleneck to their application in (1) disease modelling-as most cardiac (genetic) diseases have a middle-age onset-and (2) clinically relevant models, where integration and functional coupling are key. So far, several methods have been reported to enhance iPSC-CM maturation; however, these protocols are laborious, costly, and not easily scalable. Therefore, we developed a simple, low-cos

SUBMITTER: Chirico N 

PROVIDER: S-EPMC9795728 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature