Establishment and characterization of a new intrahepatic cholangiocarcinoma cell line derived from a Chinese patient.

ABSTRACT: Patients with intrahepatic cholangiocarcinoma (ICC) require chemotherapy due to late detection, rapid disease progression, and low surgical resection rate. Tumor cell lines are extremely important in cancer research for drug discovery and development. Here, we established and characterized a new intrahepatic cholangiocarcinoma cell line, ICC-X1. STR testing confirmed the absence of cross-contamination and high similarity to the original tissue. ICC-X1 exhibited typical epithelial morphology and formed tumor spheres in the suspension culture. The population doubling time was approximately 48 h. The cell line had a complex hypotriploid karyotype. The cell line exhibited a strong migration ability in vitro and cell inoculation into BALB/c nude mice led to the formation of xenografts. Addition

SUBMITTER: Miao X 

PROVIDER: S-EPMC9795767 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature