Comprehending the allergen repertoire of shrimp for precision molecular diagnosis of shrimp allergy.

ABSTRACT:

Background

Clinical management of shrimp allergy is hampered by the lack of accurate tests. Molecular diagnosis has been shown to more accurately reflect the clinical reactivity but the full spectrum of shrimp allergens and their clinical relevance are yet to be established. We therefore sought to comprehend the allergen repertoire of shrimp, investigate and compare the sensitization pattern and diagnostic value of the allergens in allergic subjects of two distinct populations.

Methods

Sera were collected from 85 subjects with challenge-proven or doctor-diagnosed shrimp allergy in Hong Kong and Thailand. The IgE-binding proteins of Penaeus monodon were probed by Western blotting and identified by mass spectrometry. Recombinant shrimp allergens were synthesized and analyzed f

SUBMITTER: Wai CYY 

PROVIDER: S-EPMC9795902 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature