The glucose tolerance test in mice: Sex, drugs and protocol.

ABSTRACT:

Aim

To establish the impact of sex, dosing route, fasting duration and acute habituation stress on glucose tolerance test (GTT) measurements used in the preclinical evaluation of potential glucose-modulating therapeutics.

Methods

Adult male and female C57Bl/6J mice, implanted with HD-XG glucose telemetry devices, were fasted for 16 hours or 6 hours following acute habituation stress due to whole cage change, cage change with retention of used bedding or no cage change prior to intraperitoneal (IP) GTTs. To evaluate protocol refinement and sex on the ability of the GTT to detect drug effects, we administered 250 mg/kg oral metformin or 10 nmol/kg IP exendin-4 using optimized protocols.

Results

Female mice were less sensitive to human intervention when initiating fastin

SUBMITTER: Kennard MR 

PROVIDER: S-EPMC9795999 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature