The use of isothermal titration calorimetry for the assay of enzyme activity: Application in higher education practical classes.

ABSTRACT: Determination of enzyme activity is crucial for discovery, research, and development in life sciences. The activity of enzymes is routinely determined using spectrophotometric assays that measure rates of substrate consumption or product formation. Though colorimetric-based detection systems are simple, rapid, and economical to perform, the majority of enzymes are unsuitable for this technique as their substrates/products do not absorb in the UV or visible range. This limitation can be addressed by the use of coupled-enzyme assays or artificial chromogenic substrates; however these approaches have their own drawbacks. Here, we describe a method based on the use of an isothermal titration calorimeter (ITC) to measure the heat produced or absorbed during any enzyme-catalyzed reaction. The co

SUBMITTER: Siddiqui KS 

PROVIDER: S-EPMC9796012 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature