A method based on plateletpheresis to obtain functional platelet, CD3⁺ and CD14⁺ matched populations for research immunological studies.

ABSTRACT:

Background

In previous studies with peripheral blood cells, platelet factors were found to be associated with severe allergic phenotypes. A reliable method yielding highly concentrated and pure platelet samples is usually not available for immunological studies. Plateletpheresis is widely used in the clinics for donation purposes. In this study, we designed a protocol based on plateletpheresis to obtain Platelet-Rich Plasma (PRP), Platelet-Poor Plasma (PPP) as well as CD3⁺ and CD14⁺ cells matched samples from a waste plateletpheresis product for immunological studies.

Methods

Twenty-seven subjects were voluntarily subjected to plateletpheresis. PRP, PPP and blood cell concentrate contained in a leukocyte reduction system chamber (LRSC) were obtained in

SUBMITTER: Pablo-Torres C 

PROVIDER: S-EPMC9796013 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature