Diversity of novel archaeal viruses infecting methanogens discovered through coupling of stable isotope probing and metagenomics.

ABSTRACT: Diversity of viruses infecting non-extremophilic archaea has been grossly understudied. This is particularly the case for viruses infecting methanogenic archaea, key players in the global carbon biogeochemical cycle. Only a dozen of methanogenic archaeal viruses have been isolated so far. In the present study, we implemented an original coupling between stable isotope probing and complementary shotgun metagenomic analyses to identify viruses of methanogens involved in the bioconversion of formate, which was used as the sole carbon source in batch anaerobic digestion microcosms. Under our experimental conditions, the microcosms were dominated by methanogens belonging to the order Methanobacteriales (Methanobacterium and Methanobrevibacter genera). Metagenomic analyses yielded several previo

SUBMITTER: Ngo VQH 

PROVIDER: S-EPMC9796341 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature