Prevalent and dynamic binding of the cell cycle checkpoint kinase Rad53 to gene promoters.

ABSTRACT: Replication of the genome must be coordinated with gene transcription and cellular metabolism, especially following replication stress in the presence of limiting deoxyribonucleotides. The Saccharomyces cerevisiae Rad53 (CHEK2 in mammals) checkpoint kinase plays a major role in cellular responses to DNA replication stress. Cell cycle regulated, genome-wide binding of Rad53 to chromatin was examined. Under replication stress, the kinase bound to sites of active DNA replication initiation and fork progression, but unexpectedly to the promoters of about 20% of genes encoding proteins involved in multiple cellular functions. Rad53 promoter binding correlated with changes in expression of a subset of genes. Rad53 promoter binding to certain genes was influenced by sequence-specific trans

SUBMITTER: Sheu YJ 

PROVIDER: S-EPMC9797190 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature