Humic Acids Inhibit Platelet Activation to Reduce Venous Thromboembolism in Mice.

ABSTRACT:

Objective

We aimed to investigate the effects of the natural product humic acids (HA) on platelet activation and development of venous thromboembolism (VTE) in mice and further explore the relevant mechanism.

Methods

Eight-week C57BL/6 mice were randomly assigned to three groups: sham operation group (n = 7), VTE group (n = 8), and VTE + HA group (n = 10). Thrombi were harvested to hematoxylin-eosin staining to evaluate the thrombolysis and recanalization of the thrombus. In addition, flow cytometry was performed to detect the expression levels of protein disulfide isomerase on endothelial-derived exosomes and glycoprotein IIb/IIIa on the surface of the activated platelets surface in plasma. Furthermore, the protein expression level of glycoprotein IIb/I

SUBMITTER: Lan HT 

PROVIDER: S-EPMC9797308 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature