Development of a simple multiple mutation detection system using seed-coat flavonoid pigments in irradiated Arabidopsis M₁ plants.

ABSTRACT: Ionizing radiation induces genetic variations in plants, which makes it useful for plant breeding. A theory that the induced mutations occur randomly in the genome has long been accepted, but is now controversial. Nevertheless, a comparative analysis of the mutations at multiple loci has not been conducted using irradiated M₁ genomes that contain all types of mutations. In this study, we identified Arabidopsis mutants (pab2 and pab3) in a mutagenized population of an anthocyanin-positive seed mutant (ban). Both pab2 and pab3 were revealed to be double mutants (tt4 ban and tt8 ban, respectively) that produced similar anthocyanin-less immature seeds, but differentially colored mature seeds. These features enabled the seed color-based detection of de novo M₁ mutations in

SUBMITTER: Kitamura S 

PROVIDER: S-EPMC9797493 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature